![]() Major driver of this market is the increase investment in R&D, widespread usage of northern blotting in healthcare, agriculture and pharmaceutical industry. The prevalence and incidence of cancer is increasing across the globe and expected to increase the investment in cancer related research programs which in turn, can boost the growth of Northern Blotting market over the forecast period. The major use of northern blotting technique is in cancer study.Īccording to WHO, approximately 14 Million cases of cancer were reported in the year 2012. ![]() Northern Blotting market is expected to grow with a healthy CAGR over the forecast period owing to increasing investment in pharmaceutical and biotechnology industry related to R&D procedures. These widespread application has created a significant importance for Northern Blotting technique and it is expected to boost the market growth of Northern Blotting in near future. Gene identification and expression has become an important aspect in drug discovery and development and has widespread applications in pharmacogenomics, diagnostics, toxicology etc. It is also used to identify the specific site of RNA where gene expression occurs along with identification of the gene expression patterns. Northern Blotting has widespread use in molecular and biological research to calculate the RNA size and related protein that RNA encodes. It works on DNA-RNA hybridization technique and is similar to Southern Blotting technique. Northern Blotting is a key technique in molecular biology to identify the gene and related disorder. These processes are used to understand the functions of different genes in the body and to identify the diseases and disorders associated with them. ThermoFisher Scientific (2019b) The basics: Northern analysis.Northern Blotting Market: Global Industry Analysis and Opportunity Assessment 2016-2026 OverviewĬompletion of the human genome project in 2003 has created a significant importance for the identification of gene and its expression. ThermoFisher Scientific (2019a) Overview of Western blotting. Southern E (2015) The early days of blotting. Kurien BT, Scofield RH (2006) Western blotting. ![]() Keywordsīurnette WN (1981) “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. The term ‘blotting’ in all the three techniques represents the transfer of material after separation to nitrocellulose paper by means of diffusion. Northern blotting is used to detect mRNA of interest, where after separation by electrophoresis, cDNA is used as a probe that binds to the RNA strand the application includes finding alternate transcript size. Application of Western blotting includes identifying HIV antigens or Hepatitis B surface antigen in blood. In Western blotting, this is made possible by primary and secondary antibodies, whereas in Southern blotting, a radiolabeled (fluorescent) probe or dye that binds to the DNA is used. The difference lies in the visualization process. Western blotting is the counterpart which is used to detect proteins. The steps involved are isolation of DNA, its separation by electrophoresis, transfer to a suitable medium, hybridization to probes and visualization of the gene if it is present. Southern, represents a technique to detect a gene of interest in the DNA sample. ![]() Southern blotting, discovered in 1975 by E.M. ![]()
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